Technical Services FAQs
Telephone1-301-840-93311-800-443-9331 Fax1-301-840-0743E-mail:TechnicalService@qualitybiological.comCommon Questions:The following are questions frequently addressed to Quality Biological's Customer Service and Technical Service personnel. They are provided here to assist in your choice and use of Quality Biological products for cell and molecular biology research.Cell Biology FAQ'sHow stable are your sera and basal media? What is the pH of your Cell Culture Grade water? How do I adapt my cells from a serum-containing medium to one of your serum-free media (SFM)?
Molecular Biology FAQ'sWhat is the pH of your DEPC-treated Water and Molecular Biology Grade Water? What is the difference between your SOC and SOB media? What is the difference between your Lennox L and Luria-Bertani media? What is the formulation of your TE buffers? Is your Prehybridization/Hybridization buffer qualified for RNA? How do you remove "DEPC" from your DEPC-treated water? What are the compositions of your RNA, DNA, and SDS-Protein Gel Loading Solutions? What is the formulation of your RNA Gel Buffer (10X MOPS Buffer)? Why do you not put formamide in your gel loading solutions? Cell Biology FAQ’sQuestion: Should I supplement Quality Biological serum-free media (SFM) with L-glutamine prior to use? If so, what should be the final concentration of L-glutamine? Answer: We recommend that Quality Biological SFM be supplemented with L-glutamine to a final concentration of 1-3 mM prior to use. Studies indicate that L-glutamine containing media, even when stored at +4° C, is stable for only 3 weeks. At higher temperatures, this particular amino acid is far less stable. Question: How stable are your sera and basal media? Answer: If our sera are stored properly, namely at -10 to -20° C, they should be acceptable for use up to 48 months. With the exception of DMEM, all other basal media stored at +4° C will have a 2-year expiration date. However, DMEM even at refrigerated temperatures, tends to precipitate after 1 year. Question: What is the pH of your Cell Culture Grade water? Answer: In general, water has an extremely low buffering capacity. It is not unusual for water to range in pH from 2 to 10 units. Question: How do I adapt my cells from a serum-containing medium to one of your serum-free media (SFM)? Answer: Basically, there are two ways: The "Weaning Method" and "The Quick Method." These are described in the Quality Biological Catalog. In addition, a separate document describing these two methods and other aspects of serum-free cell culture is available. Click on Serum Free Medium Cell Culture to view or print the document. Question: I am interested in your custom cell biology products. What are your minimum requirements for customized reagents? Answer: We are committed to providing the very best custom manufacturing service and we are pleased to assist with both small and large volume requirements. If the product of interest must be sterile filtered, our minimum requirement is 25L; whereas for products that can be autoclaved, our minimum is only 5L. Custom products can be packaged into 1mL to 20L size containers. Molecular Biology FAQ’sQuestion: What is the pH of your DEPC-treated Water and Molecular Biology Grade Water? Answer: In general, water has an extremely low buffering capacity. It is not unusual for water to range in pH from 2 to 10 units. Question: What is the difference between your SOC and SOB media? Answer: SOC contains 20mM glucose whereas SOB has none.
*Omitted from preparation. Add 20mM of MgCl2 prior to use.
Question: What is the difference between your Lennox L and Luria-Bertani media? Answer: The difference between these two bacteriological media is their respective sodium chloride concentrations. Lennox L broth contains 5.0 g/L NaCl as opposed to Luria-Bertani which has 10.0 g/L of sodium chloride.
Question: What is the formulation of your TE buffers? For RNA applications (e.g. Northern Blotting), we recommend our Hybridization Buffer for Nylon Membranes. Click on TIS to access the library of Technical Information Sheets for more information.
i). Migration in a polyacrylamide gel:
ii). Migration in a denaturing polyacrylamide gel:
iii). Migration in an agarose gel (Source: FMC):
Question: What is the formulation of your RNA Gel Buffer (10X MOPS Buffer)? Answer: The formulation is provided in a Technical Information sheet available from Customer Service. Alternatively, it may be viewed on-line. Click on TIS to access the library of Technical Information Sheets for more information. Question: Why do you not put formamide in your gel loading solutions? Answer: Formamide, an organic denaturant, is commonly used in nucleic acid applications such as sequencing. It is well known that formamide, even at very low temperatures (e.g., –20 ° C), will break down into formic acid and ammonia. Both by-products are capable of degrading RNA and DNA. Thus, it is prudent to use freshly deionized formamide before adding it to our DNA Gel Loading Solution and/or to our RNA Gel Loading Solution. Click on TIS to access the library of Technical Information Sheets for more information. Answer: Our agar plates are supplied in two sizes: 15 X 100mm (standard) and 15 X 150mm. Our minimum requirement is 100 plates. You may specify a fill volume; we typically do 25 and 40mL fills. In most cases, we can deliver a custom order within 5-10 business days. Answer: For reagents/solutions that are capable of being tested for sterility and for the absence of DNases and RNases, we can deliver such quality assurances to the customer. On the other hand, our cubitainer suppliers cannot guarantee sterility nor the absences of nucleases. Thus, once the quality assured product is added to a non-quality assured container (e.g., cubitainer), we can no longer guarantee sterility, etc., of the finished goods.
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