Cell Culture

Quality Biological manufactures and supplies a full range of cell culture media, including basal media like DMEM, RPMI, MEM, and Ham’s F-12. All formulations are sterile, QC-tested, and available in custom or standard formats.

To select the right cell culture products, consider the following:

• Specific cell line needs: Different cell types have varying media, serum, and supplement requirements. Check if the product is suitable for your cell line.
• Supplier reputation: Look for trusted suppliers with experience providing quality cell culture products.
• Product specifications: Review product datasheets for compatibility, concentrations, and handling instructions.
• Certificate of Analysis (COA): Evaluate how a product is QC tested and the acceptance release criteria before the product is made commercially available.

QBI offers proper shipping methods for temperature-sensitive products, such as refrigerated or frozen media and serum. Insulated packaging with dry ice or gel packs is often used to maintain these items at the correct temperature during transit. 

Always check shipping policies and timelines before placing an order, especially for temperature-sensitive materials like media or serum. A product can be shipped ambient even if its recommended storage temperature is 2-8 °C because the brief transport at ambient conditions will not impact the quality and effectiveness of the product. Consult the product page on our website to see the product storage and shipping temperatures.

Yes, all Quality Biological products are produced in a controlled limited access environment, aseptically dispensed into sterile containers, and are ready for use when you receive them.

PBS and DPBS are buffers, supplied with or without calcium and magnesium, that are used to maintain physiological pH in biological and cell culture applications.

PBS is a phosphate-buffered saline solution commonly used for washing cells before dissociation, diluting cells for counting, transporting of cells or tissue, a general-purpose buffer for reagent prep and molecular biology applications.

DPBS (Dulbecco’s Phosphate-Buffered Saline) is a specific PBS formulation that typically includes potassium salts (KCl and KH₂PO₄) and defined salt concentrations providing physiological pH. It is a balanced salt solution that serves as irrigation, transporting, or diluting fluid for cells.

Both antibiotic-free and phenol red-free options are available, as well as media with these components depending on your experimental needs.

Cell culture media should be stored at 2-8 °C.

When stored properly at 2-8 °C, most cell culture medium should have at least a 2-year shelf-life from the date of manufacture. The exception is DMEM, which has a 1-year shelf life. DMEM tends to precipitate after 12 months even if refrigerated.

Yes. We specialize in custom media development and can adjust formulation, pH, supplements, and packaging to fit your requirements. Let us know what you need by completing our Custom Formulation Request Form.

We are committed to providing the very best custom manufacturing service and we are pleased to assist with both small and large volume requirements. If the product of interest must be sterile filtered, our minimum volume requirement is 25 Liters, whereas, for products that can be autoclaved, our minimum volume is only 5 Liters. Custom cell culture media can be packaged into containers from 500 mL to 200 L.

For custom-manufactured products, please complete our Custom Formulation Information Request Form.

During shipping, the trypsin might change from pink to yellow in color due to exposure to carbon dioxide released from the dry ice in the shipping container. In our experience, this discoloration does not affect the functionality of the trypsin.