Enz-Free Solution is a proprietary, non-enzymatic solution specially formulated for the non-destructive detachment of adherent cells from culture vessel surfaces. Enz-Free is a gentle dissociation of mammalian cells for studies requiring intact cell surface proteins such as ligand binding, flow cytometry, and immunohistochemistry.
- Recommended for detaching adherent cells growing in serum free conditions
Enz-Free works well with lightly adherent cell lines such as HeLa and NIH 3T3, but is not recommended for routine passage of strongly adherent cells. Enz-Free is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in Dulbecco’s Phosphate Buffered Saline (DPBS) without calcium and magnesium.
Enz-Free is useful for serum-containing cell culture systems, serum-free cell culture systems, and protein-free cell culture systems.
Use ENZ-Free with accessory item QBSF 60 serum free cell culture medium.
Storage Conditions: 15°C to 30°C
Shipping Condition: ambient temperature
pH: 7.4 ± 0.2
Osmolality: 310±5% mOsm/Kg H20
Harmonization Code: 3822.00.5090
Max Shelf Life: 36 months