QBSF-60® Serum Free Medium

Description

QBSF-60 is a proprietary serum-free, xeno-free expansion medium that supports the expansion of CD34+ human hematopoietic stem/progenitor cells.

QBSF-60 supports CD34+ cells derived from normal bone marrow, cord blood, peripheral blood stem cells mobilized with cytokines for ex-vivo expansion and differentiation to erythrocytes, granulocytes, and lymphocytes.

• Serum-Free, Xeno-Free Medium
• High cytokine-driven expansion rate of CD34+ hematopoietic stem cells
• Contains L-Glutamine and Phenol Red
• Endotoxin, mycoplasma, and performance tested
• Sterile Filtered

Applications

QBSF-60 can be used for the maintenance of human hematopoietic stem cell culture, to support CD34 cell proliferation and differentiation to multi-lineage cells, and to support human umbilical and blood vessel endothelial cells

The QBSF products have been successfully used in different cell culture applications. Investigators are encouraged to explore new uses.

QBSF products used in various applications

• Acute Sensitivity of Ph-like Acute Lymphoblastic Leukemia to the SMAC-Mimetic Birinapant. Richmond et al., Cancer Res. 2016 Aug 1;76(15):4579-91.
• Efficient iPS cell generation from blood using episomes and HDAC inhibitors. Hubbard JJ. et al., J Vis Exp. 2014 Oct 28;(92):e52009.
• Initial CD34+ cell-enrichment of cord blood determines hematopoietic stem/progenitor cell yield upon ex vivo expansion. Andrade et al., J Cell Biochem. 2011 Jul;112(7):1822-31.
• HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding. Yu et al., Stem Cells. 2006 Apr;24(4):876-88.
• Short-term exposure of umbilical cord blood CD34+ cells to granulocyte-macrophage colony-stimulating factor early in culture improves ex vivo expansion of neutrophils. Marturana et al., Cytotherapy. 2011 Mar;13(3):366-77.
• Stromal cell-free conditions favorable for human B lymphopoiesis in culture. Ichii et al., J Immunol Methods. 2010 Jul 31;359(1-2):47-55.
• Constitutive activation of STAT5A promotes human hematopoietic stem cell self-renewal and erythroid differentiation. Schuringa et al., J Exp Med. 2004 Sep 6;200(5):623-35.
• Ex Vivo Expansion of CD34+ Umbilical Cord Blood Cells in a Defined Serum-Free Medium (QBSF-60) with Early Effect Cytokines. Qiu et al., J. Hematotherapy & Stem Cell Research, 2004 Jul 9; 8(6):609
• Evaluation of Serum-Free Culture Conditions Able to Support the Ex Vivo Expansion and Engraftment of Human Hematopoietic Stem Cells in the Human-to-Sheep Xenograft Model, Almeida-Porada et al., J. Hematotherapy & Stem Cell Research, 2024, Jul 9; 9(5):591
• Support the Ex Vivo Expansion and Engraftment of Human Hematopoietic Stem Cells in the Human-t0-Sheep Xenograft Model, Almeida-Porada et al., J. of Hematotherapy & Stem Cell Research, 2024 July 9; 9(5):683
• Grafts of adenosine-releasing cells suppress seizures in kindling epilepsy. Huber et al. Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7611-6.
• Cytosine deaminase adenoviral vector and 5-fluorocytosine selectively reduce breast cancer cells 1 million-fold when they contaminate hematopoietic cells: a potential purging method for autologous transplantation. Garcia-Sanchez et al. Blood. 1998 Jul 15;92(2):672-82.
• Adaptation of BHK cells producing a recombinant protein to serum-free media and protein-free medium. Cruz et al. Cytotechnology. 1998 Jan;26(1):59-64.
• Serum-free culture conditions for cells capable of producing long-term survival in lethally irradiated mice. Brown et al. Stem Cells. 1997;15(3):237-45.

Storage Conditions: 2°C to 8°C

Shipping Condition: ambient temperature

Sterile Filtered

pH: 7.0± 0.2

Max Shelf Life: 24 months from date of manufacture

Harmonization Code: 3821.00.0000

ECCN: EAR99