Endotoxin Free DPBS Buffer for Critical Research Applications
OVERVIEW
EndoToxFree™ DPBS (1X), is an ultra-pure, USP Grade Dulbecco’s phosphate-buffered saline, formulated without calcium and magnesium. Manufactured under stringent conditions, ensuring an endotoxin level of <0.005 EU/mL, this buffer is specifically designed for sensitive cell culture and research applications where endotoxin contamination could compromise results.
UNDERSTANDING ENDOTOXIN CONTAMINATION
What Are Endotoxins?
Endotoxins are lipopolysaccharide (LPS) components found in the outer membrane of Gram-negative bacteria. Even in trace amounts, these toxic compounds can severely disrupt cell culture experiments and research outcomes.
Sources of Endotoxin Contamination
- Equipment and Surfaces: inadequate cleaning and sterilization increases risk
- Environmental Exposure: cross-contamination during handling or storage
Research Impacts of Endotoxin Contamination
Even trace amounts of endotoxins can severely affect your experimental results.
- Unwanted Immune Cell Activation – cytokine release in immune cell lines (e.g., THP-1, RAW 264.7)
- Compromised viability and differentiation of stem cells and primary cells
- Significant batch-to-batch variability in assay results
- False positive/negative results in inflammation-related assays
- Artificial immune activation leading to unreliable data
KEY BENEFITS OF EndoToxFree™ DPBS (1X), USP Grade
- Ultra-low endotoxin levels (<0.005 EU/mL) eliminate interference that can compromise your critical experiments
- USP Grade quality ensures high purity and consistent composition from batch to batch
- Calcium and magnesium-free formulation gives precise control over cell behavior
- Reliable, reproducible results across manufacturing lots
- Regulatory compliance support for smooth transitions from research to GMP manufacturing
Calcium and Magnesium Free for Enhanced Cell Control
- Supports cell detachment – Eliminating calcium and magnesium ions prevents ion-mediated cell adhesion during cell harvesting procedures.
- Prevents Unwanted Reactions – Calcium and magnesium can activate certain enzymes or signaling pathways, potentially affecting experimental outcomes.
- Avoids Precipitation or Contamination – In some biochemical or immunological experiments, these ions might cause unwanted precipitation or interactions
RESEARCH APPLICATIONS THAT BENEFIT FROM ENDOTOXIN-FREE REAGENTS
Reproducibility, sensitivity, and accuracy of your research can be directly impacted by endotoxin contamination. EndoToxFree™ DPBS is critical for:
PERFORMANCE BENEFITS
| Feature | Research Impact |
|---|---|
| Improved Reproducibility | Consistent results across replicates and experimental batches |
| Enhanced Cell Viability | Reduced unintended immune activation and cellular stress |
| Increased Assay Sensitivity | Eliminates LPS interference in cytokine and ELISA-based assays |
| Reliable Comparisons | Critical for peer-reviewed validation and publication |
| Cost Efficiency | Minimizes repeat experiments due to contamination issues |
Product Specifications |
|---|
| Product Name | EndoToxFree DPBS (1X), USP Grade |
| Item Number | 114-348-101 |
| Volume | 500 mL |
| Endotoxin Level | <0.005 EU/mL (Guaranteed) |
| Formulation | without Calcium and Magnesium |
| Water Quality | Cell Culture, WFI Quality |
| Testing | Endotoxin level, sterility, and mycoplasma |
For Research or Further Manufacturing Use. Not for Diagnostic or Therapeutic Use.
